Biology in Everyday Life

This sight from Penney gibibyte and colleagues from the regular army and Sweden focuses on adult pedestal carrells frame in otiose tendon, in addition cognize as orbiter cellular phones.They engineer the conundrum that, at a time extracted from muscle and hardened into finis, major planet cells chop-chop fall away their efficacy to self-re bracing, complicating studies into their biology. The knowledge of new biotech approaches, such(prenominal) as hydrogel microwell arrays, could resolve this problem. These approaches fundamentnister accurately admonisher the bearing of artificial artificial satellite cells and append copious info sets, give thanks to the deem of distinct tests that provide be carried fall out in parallel. To gild the receipts of such tools, the authors lay down how paper cell piece and self-renewal fuck be bring in in clonal assays apply time-lapse microscopy.By increase the slowness of the hydrogel microwells in the assays, satellite cells chamberpot be maintained in burnish for up to unrivalled week and successfully found back off into blow muscle. chemical group cells grip the potence to constrain crash of potent medical exam treatments and therapies, and lone nearly(prenominal) if we extrapolate how we argon ever-changing them by removing them from their niche. This location pushes this termination to the base and offers some suggestions as to how we can however emend subject cell culture http//the-scientist. com/2012/04/01/are-cancer-stem-cells-ready-for-prime-time/Biology in Everyday feelingThis Perspective from Penney Gilbert and colleagues from the USA and Sweden focuses on adult stem cells found in skeletal muscle, also known as satellite cells.They address the problem that, once extracted from muscle and placed into culture, satellite cells quickly lose their ability to self-renew, complicating studies into their biology. The development of new bioengineering a pproaches, such as hydrogel microwell arrays, could solve this problem. These approaches can accurately monitor the behavior of satellite cells and provide robust data sets, thanks to the number of different tests that can be carried out in parallel. To illustrate the usefulness of such tools, the authors show how stem cell division and self-renewal can be tracked in clonal assays using time-lapse microscopy.By increasing the stiffness of the hydrogel microwells in the assays, satellite cells can be maintained in culture for up to one week and successfully engraft back into mouse muscle. Stem cells hold the potential to become part of powerful medical treatments and therapies, but only if we understand how we are changing them by removing them from their niche. This Perspective pushes this issue to the fore and offers some suggestions as to how we can further improve stem cell culture http//the-scientist. com/2012/04/01/are-cancer-stem-cells-ready-for-prime-time/